Monday, March 7, 2011

#5 Vector Cloning VS PCR

Vector Cloning

- I don't think it is relatively cheaper than PCR because if you think about what you need; plasmid, gene of interest ,yeast, specific R.E etc. Thoes are not cheap.

- replicates entire DNA and once it is replicated, you are going to get the same targeted DNA over a long period of time.

- Depending on the purpose, it can produce protien.

PCR

- Takes less time than Vector Cloning.

- It only replicates DNA.

- one DNA strand can produce a billion strands of DNA in no time, but if the one DNA strand is contaminated, then the rest of the copied DNA is also contaminated.

- it is widely used in forensics. When they gather evidence, the first thing they do is PCR.

-If there is machine for PCR, everything is automated, so you can just leave it to machine and go for lunch.

*I'll edit more if i find more info regarding this topic :D